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1. General Information | ย |
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Name of CBB | CYCORD PACBB |
CBB Director | PAUL |
CBB Director | COSTEAS |
Address | OLD ROAD NICOSIA-LIMASSOL |
Address | NICOSIA GENERAL HOSPITAL |
Address | 2029 |
Address | NICOSIA |
Phone Number | 0035722603246 |
Date CBB Started Collecting Cord Blood Units (month/day/year) | 11/19/2005 |
Number of Public Cord Blood Units | 2,634 |
Planned Number of Public Cord Blood Units Stored in 2015 | 400 |
Lists on BMDW | Yes |
Affiliated with National Stem Cell Donor Registry | Yes |
Registry Affiliation | CYPRUS BONE MARROW DONOR REGISTRY, CYBMDR |
2. Cord Blood Units in Inventory | ย |
Current Processing Method | Vapour+ red auto |
Previous Processing Method | Liquid+ red auto |
Year Current Process Method Started | 2008 |
Percent of Units Plasma and RBC Reduced (automated) | 71 |
Percent of Units No Volume Reduction | 29 |
3. Accreditations, Licenses and Certifications | ย |
FACT-Netcord | In process of accreditationYes |
AABB | No |
Competent Authority/ National Health Authority | Yes |
Name of Competent Authority | MINISTRY OF HEALTH |
Audited by National Stem Cell Registry | Yes |
ISO | No |
4. Cord Blood Collection | ย |
Current Collection Practice Is the collection In/Ex -Utero or both? | In-utero |
Current Antiseptic | Betadine |
Collection Bag | Double needle |
Agitation during Collection | None |
5. Conditioning/Transport from Collection Site to CBB | ย |
Secondary Bag Used | Yes |
Transport Conditions | Unqualified transporter |
Transport Conditions | Passive refrigeration system |
Transport Conditions | Electronic temperature probe |
Transport Conditions | Ground transport |
Temp. for Storage and Transport | Defined (above +8ยฐC) |
6. Pre-Processing Evaluation | ย |
Completed Prior to Accepting a CBU | Medical history, collection report, informed consent |
Current Threshold for Accepting a CBU | Viability threshold CD45 |
Method for CD34 Remuneration | ISHAGE guidelines |
External Proficiency Testing for QC of FACS Lab | UKNEQAS |
Post Processing/ Pre Freeze CD34+ Cell Count | Yes |
Time from Collection to Processing | up to 48H |
7. Processing and Packaging | ย |
Pre Freeze Processing Methods- Unit in Inventory | No processing |
Pre Freeze Processing Methods- Unit in Inventory | SEPAX |
Pre Freeze Processing Methods- Current | SEPAX |
Additives Currently in Use | HES |
Current Cryopreservation Method | Conventional CRF |
Current Cryoprotectant Additive | Ready for use DMSO-Dextran |
Current Cryobag | Single bag 80:20 |
Current Target Cryopreservation Volume (mL) | 27.0 |
Current Packaging for Storage | Overwrap |
Current Packaging for Storage | Canister |
Current Packaging for Storage | More than one segment |
8. Testing | ย |
Extra Material Currently Stored | Cord blood DNA |
Extra Material Currently Stored | Plasma/cord blood |
Extra Material Currently Stored | Maternal DNA |
Extra Material Currently Stored | Maternal plasma/serum |
Current Post Processing Threshold for Accepting a CBU for Public Use TNC | 100 |
Current Post Processing Threshold for Accepting a CBU for Public Use CD34 (10^6) Single Platform | 1.00 |
Current Post Processing Threshold for Accepting a CBU for Public Use CD34 (10^6) Double Platform | NA |
Current Post Processing Threshold for Accepting a CBU for Public Use CFU-GM | NA |
Current Post Processing Threshold for Accepting a CBU for Public Use CFU | NA |
Current Post Processing Threshold for Accepting a CBU for Public Use Viability | 75 |
9. Storage | ย |
Type of Storage Container Used | Conventional storage tank vapor phase |
Type of Storage Container Used | Conventional tank liquid phase |
Monitoring of Storage | Alarm on individual tanks only |
Monitoring of Storage | Centralized system remote monitoring |
Monitoring of Storage | LN2 level |
Monitoring of Storage | Lid opening |
Monitoring of Storage | Temperature monitoring |
10. HLA Typing | ย |
Current Level of HLA Typing at Time of Listing HLA-A | IR |
Current Level of HLA Typing at Time of Listing HLA-B | IR |
Current Level of HLA Typing at Time of Listing HLA-C | IR |
Current Level of HLA Typing at Time of Listing HLA-DRB1 | IR |
Current Level of HLA Typing at Time of Listing HLA-DQB1 | IR |
Current Level of HLA Typing at Time of Listing HLA-DPB1 | LR |
Accreditation of HLA Lab | EFI accredited lab |
Average Turnaround Time for Extended HLA Typing Results in days | ?10 |
Attached Segment Used for Confirmatory/ Verification Typing | Yes |
Units Listed without Attached Segment and have not been Previously Typed on Attached Segment | Yes |
Percentage of CBUs that have an Attached Segment | 75-90% |
Confirmatory/ Verification Typing on an Attached Segment is Pre-Release Requirement | No |
11. Reservation and Cancellation Policies | ย |
What Point is a CBU Reserved for a Patient | HLA typing request |
Length of Time a CBU can be Reserved in days | 90 |
Reservation Fee | No |
Reservation Cancellation Fee in Absence of Shipment Request | No |
Can Reservation be Extended | Yes |
Is a Unit Report Provided on a Unit that is Reserved for Another Patient | No |
Is TC Informed when CBU is Released | Yes |
12. Release and Shipment | ย |
Hemoglobinopathy Screening Performed Prior to Release | Yes |
Criteria to Ship a CBU Viability and Cell Count | 65 |
Criteria to Ship a CBU HLA Identity Testing | Yes |
Current Packaging for Shipment to TC | Metal canister |
Current Packaging for Shipment to TC | Overwrap |
Current Packaging for Shipment to TC | Separate segment |
Current Packaging for Shipment to TC | 2 attached segments |
Time Between Shipment Request and Sending CBU | 1-2 weeks |
Fee for Shipment Cancellation | No |
Dry Shippers Validated to Maintain Temperature of at least -150 for 48 hours Beyond Expected Arrival | Yes |
Electronic Temperature Data Logger on All Dry Shippers | Yes |
Who Selects Transport Company | Requesting transplant centre |
Shape of Transport Container | Mushroom |
13. Adverse Events Reporting | ย |
Who are S(P)EARS Reported To Competent Authority | Yes |
Who are S(P)EARS Reported To National Registry | Yes |
Who are S(P)EARS Reported To Transplant Center | Yes |
Who are S(P)EARS Reported To WMDA | Yes |
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