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Section 1 General Info

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CBB answer

The information has been reviewed in year :
Name of the cord blood bank: BARCELONA CBB
Number of cord blood units the cord blood bank plans to store for public use (unrelated patients):


Section 2 Cord Blood Units in Inventory

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CBB answer

Current processing method(s):
Plasma and RBC reduced (automatic)
Plasma reduction only
Plasma and RBC reduced (manual)
RBC reduction only
Total Nucleated Cell Count (x10E7) of your cord blood units stored for Unrelated Patients (Public Use).
< 125 : 717 (17,76 %)
125 - 149 : 975 (24,5 %)
150 - 199 : 1545 (38,27 %)
200 - 250 : 618 (15,31 %)
> 250 : 182 (4,51 %)


Section 3 Cord Blood Collection

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Current practice for collecting cord blood: In-utero
Current antiseptic: Betadine Betadine and alcohol
Collection bag: Double needle
Agitation during collection: Manual


Section 4 Conditioning and transport from Collection Centre to cord blood bank

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Secondary bag used by the cord blood bank (sealed, plastic bag or similar to avoid any leakage):
Transport conditions:
Insulating transport container
Active refrigeration system
Passive refrigeration system (gel, blocks)
Electronic temperature probe
Non-electronic temperature probe
Qualified transporter
Unqualified transporter
Air transport
Ground transport
Other,
Temperature range for storage and transportation of fresh product: Temperature between +2 to +8°C


Section 5 Evaluation

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CBB answer

Pre-processing Evaluation:
Current threshold for accepting a cord blood unit for public use in the cord blood bank:
Net weight in grams (excluding bag and anticoagulant) before processing &gt; 100 grams
TNC (10E7) before processing 150 - 199
Viability CD45 positive cells (%) 80 - 89%
Viability CD45 positive cells (method) 7AAD
Viability CD34 positive cells (%) 80 - 89%
Viability CD34 positive cells (method) 7AAD
Criteria that are completed before accepting a cord blood unit for public use in the cord blood bank:
Medical History
Collection Report
Informed Consent
Maternal IDM results
Temperature and integrity of the bag
Other,
Used method for CD34 enumeration:
The cord blood banks uses external proficiency testing for QC of the cord blood banks FACS lab: yes
The cord blood bank performs post processing/pre-freeze CD34 cell count: yes
Acceptable time from collection to processing: 12-24H &lt;24h


Section 6 Processing-Packaging

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The pre-freeze processing method(s) cord blood bank uses at any stage in time:
AXP
SEPAX
Optipress
Prepacyte
Manual- plasma and red cell reduction
RBC/plasma reduction with HES
Ficoll sedimentation
Centrifugation and volume reduction
No processing
Manual- plasma reduction only
Other,
The current pre-freeze processing method(s):
AXP
SEPAX
Optipress
Prepacyte
Manual- plasma and red cell reduction
RBC/plasma reduction with HES
Ficoll sedimentation
Centrifugation and volume reduction
No processing
Manual- plasma reduction only
Other,
Additives currently in use in addition to anticoagulants and DMSO during processing:
HES
Prepacyte
Plasmalyte
Albumin
Isotonic salt solution NaCl (saline)
No additive
Other,
Cryoprotectant additives currently in use: DMSO- Dextran
Cryopreservation method currently in use:
BioArchive
MVE 1850 Vapor freezer
Programmed cryopreservation with Air Liquid program (FREEZAL)
Programmed freezer
Two-step (equilibrium) freezing
Cryobag currently in use:
Currently used packaging when a unit is stored:
Segments currently stored with the unit by the cord blood bank:
One attached segment
Two or more attached segments
Separate segments detached from the bag, but stored with the CBU
Other samples
None


Section 7 Testing

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CBB answer

Does your cord blood bank currently store extra material?
Cord blood DNA
Cord blood material for DNA extraction
Plasma/cord blood
Maternal DNA
Maternal material for DNA extraction
Maternal plasma/serum
HLA typing at time of listing:
HLA-A High Resolution
HLA-B High Resolution
HLA-C High Resolution
HLA-DRB1 High Resolution
HLA-DRB2 High Resolution
HLA-DPB1 High Resolution


Section 8 Storage

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CBB answer

The following type(s) of storage container is currently used by the cord blood bank:
BioArchive tank
Conventional storage tank-Vapor phase
Conventional tank-Liquid phase
Double walled liquid Nitrogen
Type following type(s) of storage monitoring is currently by the cord blood bank:
Alarm on individual tanks only
Centralized system-local
Centralized system-remote monitoring
LN2 level
Lid opening
System default
Temperature monitoring
No temperature monitoring


Section 9 Adverse Events Reporting

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CBB answer

Adverse Event Reporting used by the cord blood bank:
Competent authority
Internal report
National registry
Transplant centre
WMDA

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