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The WMDA makes no representations or express or implied warranties regarding any information on this site or obtained through these links; expressly disclaims all legal liability and responsibility for accuracy, completeness, currency, suitability, validity, or usefulness or such information; and is not and will not be liable for any statements, errors, or omissions in posted information, or for any losses, injuries, or damages that arise or are alleged to arise from such information. Use of any information provided on this site does not and is not intended to create a contractual or other relationship. The information is provided for guidance only and is updated on an annual basis, CBB protocols, processes and fee structures may change in the meantime and if you have any queries please aks you Search Coordinator for the most up to date information.


Section 1 General Info

Question

CBB answer

Name of the cord blood bank: Calabria Cord Blood Bank
Number of cord blood units the cord blood bank plans to store for public use (unrelated patients): 10 at year


Section 2 Cord Blood Units in Inventory

Question

CBB answer

Current processing method(s):
Plasma and RBC reduced (automatic)checkfalse
Plasma reduction onlycheckfalse
Plasma and RBC reduced (manual)checktrue
RBC reduction onlycheckfalse
Total Nucleated Cell Count (x10E7) of your cord blood units stored for Unrelated Patients (Public Use).
< 125 :
125 - 149 :
150 - 199 : 373
200 - 250 : 475
> 250 :


Section 3 Cord Blood Collection

Question

CBB answer

Current practice for collecting cord blood: In-utero and ex-utero
Current antiseptic: Chlorhexidine betadine
Collection bag: Other
Agitation during collection: Manual


Section 4 Conditioning and transport from Collection Centre to cord blood bank

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CBB answer

Secondary bag used by the cord blood bank (sealed, plastic bag or similar to avoid any leakage): yes
Transport conditions:
Insulating transport containerchecktrue
Active refrigeration systemcheckfalse
Passive refrigeration system (gel, blocks)checktrue
Electronic temperature probecheckfalse
Non-electronic temperature probecheckfalse
Qualified transporterchecktrue
Unqualified transportercheckfalse
Air transportcheckfalse
Ground transportchecktrue
Other, checkfalse
Temperature range for storage and transportation of fresh product: Temperature between +2 to +8°C


Section 5 Evaluation

Question

CBB answer

Pre-processing Evaluation:
Current threshold for accepting a cord blood unit for public use in the cord blood bank:
Net weight in grams (excluding bag and anticoagulant) before processing 80 - 89.99 grams
TNC (10E7) before processing 150 - 199
Viability CD45 positive cells (%) 80 - 89%
Viability CD45 positive cells (method) 7AAD
Viability CD34 positive cells (%) 80 - 89%
Viability CD34 positive cells (method) 7AAD
Criteria that are completed before accepting a cord blood unit for public use in the cord blood bank:
Medical Historychecktrue
Collection Reportchecktrue
Informed Consentchecktrue
Maternal IDM resultschecktrue
Temperature and integrity of the bagchecktrue
Other, checkfalse
Used method for CD34 enumeration: ISHAGE
The cord blood banks uses external proficiency testing for QC of the cord blood banks FACS lab: yes
The cord blood bank performs post processing/pre-freeze CD34 cell count: yes
Acceptable time from collection to processing: 24-48H 72 h only in specific cases (dedicate)


Section 6 Processing-Packaging

Question

CBB answer

The pre-freeze processing method(s) cord blood bank uses at any stage in time:
AXPcheckfalse
SEPAXcheckfalse
Optipresscheckfalse
Prepacytecheckfalse
Manual- plasma and red cell reductionchecktrue
RBC/plasma reduction with HEScheckfalse
Ficoll sedimentationcheckfalse
Centrifugation and volume reductionchecktrue
No processingcheckfalse
Manual- plasma reduction onlycheckfalse
Other, checkfalse
The current pre-freeze processing method(s):
AXPcheckfalse
SEPAXcheckfalse
Optipresscheckfalse
Prepacytecheckfalse
Manual- plasma and red cell reductionchecktrue
RBC/plasma reduction with HEScheckfalse
Ficoll sedimentationcheckfalse
Centrifugation and volume reductionchecktrue
No processingcheckfalse
Manual- plasma reduction onlycheckfalse
Other, checkfalse
Additives currently in use in addition to anticoagulants and DMSO during processing:
HEScheckfalse
Prepacytecheckfalse
Plasmalytecheckfalse
Albuminchecktrue
Isotonic salt solution NaCl (saline)checktrue
No additivecheckfalse
Other, checkfalse
Cryoprotectant additives currently in use: DMSO
Cryopreservation method currently in use:
BioArchivecheckfalse
MVE 1850 Vapor freezercheckfalse
Programmed cryopreservation with Air Liquid program (FREEZAL)checktrue
Programmed freezercheckfalse
Two-step (equilibrium) freezingcheckfalse
Cryobag currently in use: Single bag (one fraction)
Currently used packaging when a unit is stored: Metal canister and overwrap
Segments currently stored with the unit by the cord blood bank:
One attached segmentcheckfalse
Two or more attached segmentscheckfalse
Separate segments detached from the bag, but stored with the CBUchecktrue
Other samplescheckfalse
Nonecheckfalse


Section 7 Testing

Question

CBB answer

Does your cord blood bank currently store extra material?
Cord blood DNAchecktrue
Cord blood material for DNA extractionchecktrue
Plasma/cord bloodchecktrue
Maternal DNAcheckfalse
Maternal material for DNA extractioncheckfalse
Maternal plasma/serumchecktrue
HLA typing at time of listing:
HLA-A High Resolution
HLA-B High Resolution
HLA-C Not performed
HLA-DRB1 Not performed
HLA-DRB2 Not performed
HLA-DPB1 Not performed


Section 8 Storage

Question

CBB answer

The following type(s) of storage container is currently used by the cord blood bank:
BioArchive tankcheckfalse
Conventional storage tank-Vapor phasecheckfalse
Conventional tank-Liquid phasechecktrue
Double walled liquid Nitrogencheckfalse
Type following type(s) of storage monitoring is currently by the cord blood bank:
Alarm on individual tanks onlycheckfalse
Centralized system-localchecktrue
Centralized system-remote monitoringchecktrue
LN2 levelchecktrue
Lid openingcheckfalse
System defaultcheckfalse
Temperature monitoringchecktrue
No temperature monitoringcheckfalse


Section 9 Adverse Events Reporting

Question

CBB answer

Adverse Event Reporting used by the cord blood bank:
Competent authoritychecktrue
Internal reportchecktrue
National registrychecktrue
Transplant centrechecktrue
WMDAchecktrue

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